Abstract
Live imaging of human or other mammalian cells at multi-hour time scales with minimal perturbation to their growth state requires that the specimen’s optimal growth conditions are met while fixed to a microscope stage. In general, ideal conditions include culturing in complete growth media, an ambient temperature of 36-37 C, and a humidity-controlled atmosphere comprising typically 5-7% CO2. Commercially available devices that achieve these conditions are not a financially viable option for many labs, with the price ranging anywhere from $12000 to $40000. The advent of 3D printing technology has allowed for low-cost rapid prototyping with precision comparable to traditional fabrication methods, opening the possibility for in-lab design and production of otherwise prohibitively expensive equipment such as stage-top incubation devices. The continued usefulness and widespread availability of single-board computers (SBC) such as Arduino and Raspberry Pi also simplify the process by which these devices can be controlled. Here we report the production of a do-it-yourself (DIY) device for stage-top incubation with temperature and atmospheric control with a cost reduction of approximately 100x.
Publisher
Cold Spring Harbor Laboratory
Reference8 articles.
1. C.-P. Segeritz and L. Vallier , Cell culture, in Basic Science Methods for Clinical Researchers (Elsevier, 2017) pp. 151–172.
2. A quick guide to light microscopy in cell biology
3. Heating System Slide/Dish – Silver Line, ibidi GmbH, Lochhamer Schlag 11, 82166, Gräfelfing, Germany.
4. Arduinobased, low-cost imaging incubator for extended live cell imaging;Applied Optics,2022
5. F. C. Ay , N. Bilici , R. Varol , A. Yilmaz , U. G. Kirabali , A. Yilmaz , and H. Uvet , Study on the concept and development of a mobile incubator (2022).