Evaluation ofHOTAIR,HOXD8,HOXD9,HOXD11Gene Expression Levels in Turkish Patients with Acute Myeloid Leukemia and Chronic Myeloid Leukemia: A Single Center Experience

Abstract

ABSTRACTBACKGRAUNDHomeobox (HOX) transcript antisense RNA (HOTAIR) andHOXgenes are reported to be more expressed in various cancers in humans in recent studies. The role ofHOTAIRandHOXDgenes in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) is not well known.MATERIALS AND METHODSIn this study, expression levels ofHOXD8,HOXD9andHOXD11fromHOXDgene family andHOTAIRwere determined from peripheral blood samples of 30 AML and 30 CML patients and 20 healthy volunteers by quantitative Real Time PCR.RESULTSWe determined that the expression levels ofHOXD9andHOXD11in the AML patients were significantly lower than the control group (p<0.001 and p=0.002, respectively). There was no significant difference in the expression levels ofHOTAIRandHOXD8when compared to the control group. In the CML patients there was a significant increase in the expression level ofHOTAIRwhen compared to the control group (p=0.002). The expression levels ofHOXD9andHOXD11were found to be significantly lower than the control group (p<0.001).CONCLUSIONOur study showed thatHOTAIRmay not be a biomarker in the diagnosis and is not significantly correlated with the clinicopathological prognostic characteristics of AML. Additionally; it can be said thatHOTAIRis oncogenic by suppressing the expression ofHOXD9andHOXD11but notHOXD8in CML patients. The expression profiles ofHOTAIRmay be a potential biomarker in the diagnosis of CML patients in predicting and monitoring drug resistance.