A collection of diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producingE. coli

Abstract

SUMMARYNovel solutions are needed to reduce the risk of transmission of extended spectrum β-lactamase and AmpC β-lactamase producingEscherichia coli(ESBL/AmpCE. coli) in livestock to humans. Since phages are promising biocontrol agents, we established a collection of 28 phages against ESBL/AmpCE. coliand showed by whole genome sequencing that all phages were unique and could be assigned to 15 different genera. Host range analysis showed that 82% of 198 strains, representing the genetic diversity of ESBL/AmpCE. coli, were sensitive to at least one phage. Identifying receptors used for initial binding experimentally as well asin silicopredictions, allowed us to combine phages into two different cocktails with broad host range targeting diverse receptors. These phage cocktails inhibit growth and kill ESBL/AmpCE. coli in vitro, thus suggesting the potential of phages as promising biocontrol agents.HIGHLIGHTS28 unique phages infecting ESBL/AmpCE. coliwere isolated and characterizedBroad host range phages targeting different receptors were used to compose phage cocktailsPhage cocktails efficiently inhibit growth of ESBL/AmpCE. coli in vitro