Sex-Specific DNA Methylation and Gene Expression Changes in Mouse Placentas After Early Preimplantation Alcohol Exposure

Author:

Legault Lisa-Marie,Breton-Larrivée Mélanie,Filion-Bienvenue Fannie,Lemieux Anthony,Langford-Avelar Alexandra,Dupas Thomas,McGraw Serge

Abstract

ABSTRACTThe placenta is vital for proper embryonic development and successful pregnancy. Preimplantation alcohol exposure can negatively impact embryonic development and lead to fetal alcohol spectrum disorder. We and others have demonstrated the adverse effects of preimplantation alcohol exposure on embryo morphology and molecular profiles at late gestation, but studies on the placenta are markedly lacking. Here, we used an established preclinical mouse model to assess the impact of preimplantation (8-cell stage) binge-like alcohol exposure on the morphology, DNA methylation, and gene expression patterns in male and female late-gestation placentas. While overall placental morphology was not affected, we uncovered numerous differentially methylated regions (DMRs;n=991 in males;n=1309 in females) and differentially expressed genes (DEGs;n=1046 in males;n=340 in females) in the placentas. Remarkably, only 21 DMRs and 54 DEGs were common to both sexes, which were enriched for genes involved in growth factor response pathways. Using LASSO regression, we were able to precisely discriminate control and ethanol-exposed placentas based on their specific DNA methylation patterns. This is the first study demonstrating that preimplantation alcohol exposure alters the DNA methylation and transcriptomic profiles of late-gestation placentas. Importantly, it reveals a placental DNA methylation signature that can detect prenatal alcohol exposure and provide appropriate support to prevent eventual impact.GRAPHICAL ABSTRACT

Publisher

Cold Spring Harbor Laboratory

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3