Abstract
ABSTRACTThePectobacteriaceaefamily comprises plant pathogens able to provoke diverse diseases, including plant maceration due to the production of pectinases disrupting the plant cell wall. To better understand their natural diversity, a survey of pectinolytic bacteria was performed in lakes of the French region La Camargue near the Mediterranean Sea. Sixteen atypical pectinolytic isolates were obtained from brackish water of three lakes. The genome of six isolates was sequenced; their size is around 4.8 to 5.0 Mb, including a plasmid of 59 to 61 kb; their G+C values range from 49.1 to 49.3 mol%. Phylogenetic analyses indicated that the novel strains formed a new clade ofPectobacteriaceae, separate from previously described genera of this family. These analyses suggested also thatAcerihabitansdoes not belong toPectobacteriaceaeand should be reclassified in theBruguierivoracaceaefamily, whileSymbiopectobacteriumcould be a truePectobacteriaceaemember. Based on phenotypic, genomic and phylogenetic characteristics, we propose the creation of a new genus with the nameProdigiosinellagen. nov. Both the phenotypic and phylogenetic analyses separated the strains into two distinct subgroups. However, the DNA–DNA relatedness values revealed a close relationship between the two groups, supporting their appurtenance to the same species. Thus, it is proposed to classify them as two subspecies ofProdigiosinella aquatilissp. nov., for which we propose the nameProdigiosinella aquatilissubsp.aquatilisssp. nov. (LS101T= CFBP 8826T= LMG 32072T) andProdigiosinella aquatilissubsp.natabilisssp. nov. (CE70T= CFBP 9054T= LMG 32867T).
Publisher
Cold Spring Harbor Laboratory