Mycobacterium ulceranslow infectious dose and atypical mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer

Abstract

AbstractAddressing the transmission enigma of the neglected disease Buruli ulcer (BU) is a World Health Organization priority. In Australia, we have observed an association between mosquitoes harboring the causative agent,Mycobacterium ulcerans, and BU. Here we tested a contaminated skin model of BU transmission by dipping the tails from healthy mice in cultures of the causative agent,Mycobacterium ulcerans. Tails were exposed to mosquito (Aedes notoscriptusandAedes aegypti) blood feeding or punctured with sterile needles. Two of 11 of mice withM. ulceranscontaminated tails exposed to feedingA. notoscriptusmosquitoes developed BU. Eighteen of 20 mice subjected to contaminated tail needle puncture developed BU. Mouse tails coated only in bacteria did not develop disease. We observed a low infectious dose-50 of four colony-forming units and a median incubation time of 12 weeks, consistent with data from human infections. We have uncovered a highly efficient and biologically plausible atypical transmission mode of BU via natural or anthropogenic skin punctures.Author summaryBuruli ulcer is a neglected tropical disease caused by infection withMycobacterium ulcerans. Unfortunately, how people contract this disease is not well understood. Here we show for the first time using experimental infections in mice that a very low dose ofM. ulceransdelivered beneath the skin by a minor injury caused by a blood-feeding insect (mosquito) or a needle puncture is sufficient to cause Buruli ulcer. This research provides important laboratory evidence to advance our understanding of Buruli ulcer disease transmission.