Transport Dynamics of MtrD: an RND Multidrug Efflux Pump from Neisseria gonorrhoeae

Abstract

AbstractThe MtrCDE system confers multidrug resistance to Neisseria gonorrhoeae, the causative agent of gonorrhea. Using free and directed Molecular Dynamics (MD) simulations, we analyzed the interactions between MtrD and Azithromycin, a transport substrate of MtrD and a last-resort clinical treatment for multidrug resistant gonorrhea. We then simulated the interactions between MtrD and Streptomycin, an apparent non-substrate of MtrD. Using known conformations of MtrD homologues, we simulated a potential dynamic transport cycle of MtrD using Targeted MD techniques (TMD), and we note that forces were not applied to ligands of interest. In these TMD simulations, we observed the transport of Azithromycin and the rejection of Streptomycin. In an unbiased, long-timescale simulation of AZY-bound MtrD, we observed the spontaneous diffusion of Azithromycin through the periplasmic cleft. Our simulations show how the peristaltic motions of the periplasmic cleft facilitate the transport of substrates by MtrD. Our data also suggest that multiple transport pathways for macrolides may exist within the periplasmic cleft of MtrD.