Differential bioactivity of four BMP-family members as function of biomaterial stiffness

Author:

Sales Adrià,Khodr Valia,Machillot Paul,Fourel Laure,Guevara-Garcia Amaris,Migliorini Elisa,Albigès-Rizo CorinneORCID,Picart CatherineORCID

Abstract

ABSTRACTWhereas soft biomaterial is not able to induce cell spreading, BMP-2 presented by a soft film has been described to be sufficient to trigger cell spreading, migration and downstream BMP-2 signaling. Based on thin polyelectrolyte films of controlled stiffness, we investigated whether the presentation of four BMP members (2, 4, 7, 9) in a matrix-bound manner may differentially impact cell adhesion and bone differentiation of skeletal progenitors. We performed high content and automated screening of cellular responses, including cell number, cell spreading area, SMAD phosphorylation and alkaline phosphatase activity. The basolateral presentation of the different BMPs allowed us to discriminate the specificity of cellular response and the role of BMP receptors type I, type II, as well as three β integrins, in a BMP type and stiffness-dependent manner.

Publisher

Cold Spring Harbor Laboratory

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Engineering Surfaces with Immune Modulating Properties of Mucin Hydrogels;ACS Applied Materials & Interfaces;2022-08-24

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