Author:
Díaz Carlota Farré,Kabrani Eleni,Winkler Wiebke,Salomon Claudia,Wunderlich F. Thomas,Janz Martin,Rajewsky Klaus
Abstract
AbstractModeling complex (patho)physiological processes by sequential mutagenesis in mice is limited by the lack of optimized genetic tools and complex breeding strategies. We present a new Cre/DreERT2 dual-recombinase germinal center B-cell (GCBC)- specific strain, with co-expression of the recombinases from a single allele. This enables highly efficient Cre-mediated FOXO1 knockout followed by time-controlled, efficient Dre-mediated FOXO1 re-expression and functional rescue in GCBCs, demonstrating suitability for precise targeted sequential mutagenesisin vivo.
Publisher
Cold Spring Harbor Laboratory