Author:
Torisawa Takayuki,Saito Kei,Furuta Ken’ya,Kimura Akatsuki
Abstract
SummaryInside cells, molecular motors transport cargoes within the actively fluctuating environment known as the cytoplasm. How fluctuations in the cytoplasm affect motor-driven transport is not fully understood. In this study, we investigated the role of fluctuations for transport along microtubules usingC. elegansearly embryos, focusing on transport driven by cytoplasmic dynein. An artificial motor-cargo complex showed faster transportin vivothanin vitro, suggesting anin vivoacceleration mechanism. We also found that endogenous early endosome transport by dynein is significantly enhanced by the fluctuations in the cytoplasm, which is attributed to the activity of actomyosin networks. Anin vitroforce measurement of dynein suggests that the asymmetric force response would play a key role in the acceleration. This study provides insights into a regulatory mechanism of molecular motors within actively fluctuating cytoplasm, potentially utilizing random force originating from fluctuating dynamics in the cytoplasm to increase transport efficiency.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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