Abstract
ABSTRACTMistranslation is the misincorporation of an amino acid into a polypeptide. Mistranslation has diverse effects on multicellular eukaryotes and is implicated in several human diseases. InDrosophila melanogaster, a serine transfer RNA (tRNA) that misincorporates serine at proline codons (P→S) affects male and female flies differently. The mechanisms behind this discrepancy are currently unknown. Here, we compare the transcriptional response of male and female flies to P→S mistranslation to identify genes and cellular processes that underlie sex-specific differences. Both males and females downregulate genes associated with various metabolic processes in response to P→S mistranslation. Males downregulate genes associated with extracellular matrix organization and response to negative stimuli such as wounding, whereas females downregulate aerobic respiration and ATP synthesis genes. Both sexes upregulate genes associated with gametogenesis, but females also upregulate cell cycle and DNA repair genes. These observed differences in the transcriptional response of male and female flies to P→S mistranslation have important implications for the sex-specific impact of mistranslation on disease and tRNA therapeutics.ARTICLE SUMMARYProline-to-serine mistranslation affects male and female flies differently, but the mechanisms underlying this discrepancy are unknown. We present a transcriptomic analysis of male and female flies showing that mistranslation disrupts metabolic pathways and gametogenesis in both sexes, whereas processes such as DNA repair and cell cycle regulation are affected only in one sex. This is the first analysis that characterizes sex-specific effects of mistranslation and provides intriguing avenues for future research to understand how mistranslation affects males and females.
Publisher
Cold Spring Harbor Laboratory