Author:
Ramesan Shwathy,Mason Joel,Swanson Molly E.V.,Mills Samuel,Turner Bradley J.,Rutar Matthew,Gonsalvez David G.,Barton Samantha K.
Abstract
AbstractWe engineered a novel human iPSC-derived organoid system enriched with mature, myelinating oligodendrocytes and functionally reactive microglia. This model enables the study of human CNS remyelination following a demyelinating insult, encapsulating: myelin fragmentation, microglial clearance of myelin debris and oligodendrocyte genesis, differentiation, and axon ensheathment. This system provides a powerful and unparalleled capacity to interrogate complex human cell behaviour, relevant to those studying glial biology and neurological disease mechanisms.
Publisher
Cold Spring Harbor Laboratory