Molecular Characterization and Antibiotic Resistance Profile ofEscherichia coliIsolated From Liver Abscess

Abstract

AbstractBackgroundBacterial liver abscess is the most common hepatic infection, which can lead to death.Escherichia coliis among the many species of bacteria that cause it. This study was conducted to isolateE. colifrom liver abscess and then to characterise the bacteria’s molecular makeup and antibiotic resistance profile.MethodsA total of 208 stool samples were collected from patients showing symptoms of liver abscess.E. coliwas isolated from these samples followed by identification by biochemical tests. Pure and biochemically positive colonies were confirmed by polymerase chain reaction. The disk diffusion method was used to ascertain the pattern of antibiotic resistance exhibited byE. coliisolates.ResultsThe PCR amplification efficiency was nearly 100% since all of the samples appeared at 284 molecular base pairs (bp), which is considered to be the optimal parameter assay. The antimicrobial susceptibility pattern showed that isolates were resistant to many drugs but 100% and 92% of the isolates were susceptible to imipenem and azithromycin, respectively. All isolates were resistant to ampicillin, vancomycin, and cefotaxime. This was followed by ceftazidime (72%), tetracycline (84%), trimethoprim (80%), streptomycin (96%), linezolid (92%), Teicoplanin (80%), nalidixic acid (84%), ciprofloxacin (92%), and chloramphenicol (72%).ConclusionMultiple drug resistantE. coliis one of the causes of liver abscesses in humans.