Genetic ablation of GABABreceptors from oligodendrocyte precursor cells protects against demyelination in the mouse spinal cord

Abstract

AbstractGABAergic signaling and GABABreceptors play crucial roles in regulating the physiology of oligodendrocyte-lineage cells, including their proliferation, differentiation, and myelination. Therefore, they are promising targets for studying how spinal oligodendrocyte precursor cells (OPCs) respond to injuries and neurodegenerative diseases like multiple sclerosis. Taking advantage of the temporally controlled and cell-specific genetic removal of GABABreceptors from OPCs, our investigation addresses their specific influence on OPC behavior in the gray and white matter of the mouse spinal cord. Our results show that while GABABreceptors do not significantly alter OPC cell proliferation and differentiation under physiological conditions, they distinctly regulate the Ca2+signaling of OPCs. In addition, we investigate the impact of OPC-GABABreceptors in two models of toxic demyelination, namely the cuprizone and the lysolecithin models. The genetic removal of OPC-GABABreceptors protects against demyelination and oligodendrocyte loss. Additionally, we observe enhanced resilience to cuprizone-induced pathological alterations in OPC Ca2+signaling. Our results provide valuable insights into the potential therapeutic implications of manipulating GABABreceptors in spinal cord OPCs and deepen our understanding of the interplay between GABAergic signaling and spinal cord OPCs, providing a basis for future research.