Toxoplasma gondiiinfection accelerates the progression of hereditary spastic paraplegia

Abstract

AbstractThe parasitic protozoaToxoplasma gondiichronically infects the central nervous system of an estimated one-third of the human population. Infection is generally subclinical, but immunocompromised individuals can experience a variety of neurological symptoms. Meta-analyses ofT. gondiiseropositivity have suggested a correlation betweenT. gondiiinfection and neurologic disease. While mechanistic studies on the relationship betweenT. gondiiinfection and neurologic disease have been attempted in mice, mice are particularly susceptible toT. gondii, making them an effective model for investigating mechanisms of infection, but not ideal for examining the relationship between long-term chronicT. gondiiinfection and neurologic disease. Rats more closely mimic human clearance ofT. gondiiafter acute infection, but a lack of rat models of neurologic disease has limited studies on the interplay betweenT. gondiiinfection and neurologic disease progression. We have employed a previously characterized rat model of a complex form of hereditary spastic paraplegia (HSP), a class of neurodegenerative disorders which cause axonal degeneration and lower limb spasticity, in order to assess the effect of chronicT. gondiiinfection on neurodegenerative disease. We find that infected rats with hereditary spastic paraplegia exhibit significantly exacerbated behavioral and neuromorphological HSP symptoms compared to uninfected HSP mutant rats, with little correlative effect in infected versus uninfected control animals. We further find that all infected rats regardless of genotype exhibit a robust immune response toT. gondiiinfection, effectively clearing the parasite below the limit of detection of multiple assays of parasitemia and exhibiting no detectable increase in neuroinflammation seven weeks post-infection. These results suggest that chronic undetectedT. gondiiinfection may exacerbate neurodegenerative disease even in immunocompetent individuals and may contribute to neurodegenerative disease heterogeneity.Author SummaryThe long-term consequences of previous acute infections are poorly understood, but are becoming increasingly appreciated, particularly in the era of long Covid. Altered progression of other diseases later in life may be among the long-term consequences of previous infections. Here we investigate the relationship between previous infection with the parasiteToxoplasma gondii, which infects ∼30% of the global population, and neurodegenerative disease using a rat model of hereditary spastic paraplegia (HSP). We find that previous infection withT. gondiiaccelerates motor dysfunction in HSP rats, despite robust clearance of the parasite by infected rats. Our results suggest that previously cleared infections may alter the progression of other diseases later in life and contribute to neurodegenerative disease heterogeneity.