Abstract
AbstractFusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc), is considered one of the most destructive diseases of bananas. Paradoxically, knowledge of the genetics of the pathogen population in the Americas is very limited. In this study, a collection of 178 monosporic isolates from several banana producing regions, located in different climatic zones along a South to North transect in Brazil, was formed to assess the genetic structure of the population of Foc. The isolates underwent pathogenicity tests, PCR diagnosis for the detection of Tropical race 4 and screening to SIX homologs. The VCG of 119 isolates was determined by pairing against 17 testers. A group of 158 isolates was selected for microsatellite genotyping. There was moderate diversity of Foc in Brazil. Eight VCGs were identified: 0120, 0122, 0124, 0125, 0128, 01215, 01220, and 01222, of which 78% of isolates belong to a single VCG, while 22% of isolates belong to complexes of VCGs. The distribution of VCGs is uneven and independent of the banana genotype. VCGs were correlated with homologs of the SIX genes and varied according to geographic regions. Four SSR loci were polymorphic and on average 7.5 alleles were detected per locus. Thirty-five multilocus genotypes (MLGs) were identified. There was no association between VCG and MLGs and no genetic structure of the population of Foc in Brazil was detected.
Publisher
Cold Spring Harbor Laboratory
Reference48 articles.
1. The effector SIX8 is required for virulence of Fusarium oxysporum f.sp. cubense tropical race 4 to Cavendish banana;Fungal Biol.,2019
2. Acuña, R. , Rouard, M. , Leiva, A. M. , Marques, C. , Olortegui, A. , Ureta, C. , Cabrera-Pintado, R. M. , Rojas, J. C. , López, D. , Cenci, A. , Cuellar, W. J. , and Dita, M. 2021. First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru. Plant Dis. Available at: http://dx.doi.org/10.1094/PDIS-09-21-1951-PDN.
3. Simple sequence repeat markers for species in the Fusarium oxysporum complex
4. Understanding strain diversity in Fusarium oxysporum f. sp. cubense and history of introduction of “tropical race 4” to better manage banana production;Acta Hortic.,2009
5. Carvalhais, L. C. , Henderson, J. , Rincon-Florez, V. A. , O’Dwyer, C. , Czislowski, E. , Aitken, E. A. B. , and Drenth, A. 2019. Molecular diagnostics of banana fusarium wilt targeting secreted-in-xylem genes. Front. Plant Sci. 10.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献