Author:
Zhou Yingyan,Majluf-Cruz Abraham,Dennis Jaclyn,Woroch Eric,Hor Lilian,Hellbusch Brandon,Archuleta Erin,Fornis Lorelenn,Garcia Cindy,Aerts Shanae L.,Bai Xiyuan,Bevers Shaun,Schmidt Eric P.,Bates Melanie,Fugit Randolph V.,Nieto-Martinez Sandra,Galvan Manuel,Giclas Patricia,Frazer-Abel Ashley,Chan Edward D.
Abstract
ABSTRACTRationaleHereditary angioedema (HAE) is a potentially life-threatening illness most commonly due to deficiency or dysfunction of C1-esterase inhibitor (C1-INH). While specific treatments are available to thwart acute exacerbations, they are extremely costly and some can be associated with rare but serious side effects. The heparins are long known to augment C1-INH activity and case reports / series have documented their efficacy in treating HAE.Objectiveto determine if unfractionated heparin and two low-molecular weight heparins (enoxaparin and nadroparin) can augment C1-INH activityex vivoin the sera of patients with HAE and in anin vitrobiochemical assay.MethodsC1-INH activity in the absence or presence of the heparin formulations were analyzed by two different methods. To measure C1-INH activityex vivo, a commercially available assay was utilized with patient sera, excess amounts of C1s, and a substrate of C1s which, upon cleavage by C1s, produces a chromogenic product. To determine biochemically the C1-INH activityin vitro, a pharmacologic grade C1-INH, recombinant C1s (C1s-CCP12SP), and a peptide substrate of C1s were employed. Microscale thermophoresis was used to determine whether C1-INH binds to heparin.Main resultsin patient sera, nadroparin was superior to enoxaparin and unfractionated heparin in augmenting C1-INH activity, followed by enoxaparin and then unfractionated heparin. In thein vitrobiochemical assay, all three heparins augmented C1-INH-C1s binding linearly in a dose-dependent fashion. Microscale thermophoresis assay demonstrated that nadroparin binds to C1-INH, providing a mechanism by which heparin facilitates the interaction between C1-INH and the proteases known to produce bradykinin, the mediator of HAE.Conclusionlow-molecular weight heparin augments C1-INH activity and should be studied as a potential treatment for acute HAE.
Publisher
Cold Spring Harbor Laboratory