Author:
Chi Xiangyang,Yan Renhong,Zhang Jun,Zhang Guanying,Zhang Yuanyuan,Hao Meng,Zhang Zhe,Fan Pengfei,Dong Yunzhu,Yang Yilong,Chen Zhengshan,Guo Yingying,Zhang Jinlong,Li Yaning,Song Xiaohong,Chen Yi,Xia Lu,Fu Ling,Hou Lihua,Xu Junjie,Yu Changming,Li Jianmin,Zhou Qiang,Chen Wei
Abstract
AbstractThe pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a global public health threat. Most research on therapeutics against SARS-CoV-2 focused on the receptor binding domain (RBD) of the Spike (S) protein, whereas the vulnerable epitopes and functional mechanism of non-RBD regions are poorly understood. Here we isolated and characterized monoclonal antibodies (mAbs) derived from convalescent COVID-19 patients. An mAb targeting the N-terminal domain (NTD) of the SARS-CoV-2 S protein, named 4A8, exhibits high neutralization potency against both authentic and pseudotyped SARS-CoV-2, although it does not block the interaction between angiotensin-converting enzyme 2 (ACE2) receptor and S protein. The cryo-EM structure of the SARS-CoV-2 S protein in complex with 4A8 has been determined to an overall resolution of 3.1 Angstrom and local resolution of 3.4 Angstrom for the 4A8-NTD interface, revealing detailed interactions between the NTD and 4A8. Our functional and structural characterizations discover a new vulnerable epitope of the S protein and identify promising neutralizing mAbs as potential clinical therapy for COVID-19.
Publisher
Cold Spring Harbor Laboratory
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