Author:
Crane Adele,Abaidoo Joy,Beltran Gabriella,Fry Danielle,Furey Colleen,Green Noe,Johal Ravneet,La Rosa Bruno,Jimenez Catalina Lopez,Luong Linh,Maag Garett,Porche Jade,Reyes Lauren,Robinson Aspen,Sabbara Samantha,Herrera Lucia Soto,Negrete Angelica Urquidez,Wilson Pauline,Geiler-Samerotte Kerry,Pfeifer Susanne P.
Abstract
AbstractPhages infecting bacteria of the genus Staphylococcus play an important role in their host’s ecology and evolution. On one hand, horizontal gene transfer from phage can encourage the rapid adaptation of pathogenic Staphylococcus enabling them to escape host immunity or access novel environments. On the other hand, lytic phages are promising agents for the treatment of bacterial infections, especially those resistant to antibiotics. As part of an ongoing effort to gain novel insights into bacteriophage diversity, we characterized the complete genome of the Staphylococcus bacteriophage Metroid, a cluster C phage with a genome size of 151kb, encompassing 254 predicted protein-coding genes as well as 4 tRNAs. A comparative genomic analysis highlights strong similarities – including a conservation of the lysis cassette – with other Staphylococcus cluster C1 bacteriophages, several of which were previously characterized for therapeutic applications.
Publisher
Cold Spring Harbor Laboratory
Reference26 articles.
1. Classification of the bacteriophages of gram-positive cocci: Micrococcus, Staphylococcus, and Streptococcus;Pathol. Biol.,1975
2. Comparison of Staphylococcus phage K with close phage relatives commonly employed in phage therapeutics;Antibiotics (Basel),2018
3. Brandis H , Lenz W . 1984. Staphylokokken-Bakteriophagen. In: Meyer, W . (Ed.), Staphylokokken und Staphylokokken-Erkrankungen. VEB Gustav Fischer Verlag, Jena, pp. 186–214.
4. Characterization of Novel Phages Isolated in Coagulase-Negative Staphylococci Reveals Evolutionary Relationships with Staphylococcus aureus Phages