Nicotine affects protein complex rearrangement in Caenorhabditis elegans cells

Author:

Sobkowiak Robert,Zielezinski Andrzej,Karlowski Wojciech M.,Lesicki Andrzej

Abstract

AbstractNicotine may affect cell function by rearranging protein complexes. We aimed to determine nicotine-induced alterations of protein complexes in Caenorhabditis elegans cells, thereby revealing links between nicotine exposure and protein complex modulation. We compared the proteomic alterations induced by low and high nicotine concentrations (0.01 mM and 1 mM) with the control (no nicotine) in vivo by using mass spectrometry (MS)-based techniques, specifically the CTAB discontinuous gel electrophoresis coupled with liquid chromatography (LC-MS/MS and spectral counting. As a result, we identified dozens of C. elegans proteins that are present exclusively or in higher abundance in either nicotine-treated or untreated worms. Based on these results, we report a network that captures the key protein components of nicotine-induced protein complexes and speculate how the different protein modules relate to their distinct physiological roles. Using functional annotation of detected proteins, we hypothesize that the identified complexes can modulate the energy metabolism and level of oxidative stress. These proteins can also be involved in modulation of gene expression and may be crucial in Alzheimer’s disease. The findings reported in our study reveal intracellular interactions of many proteins with cytoskeleton and may contribute to the understanding of the mechanisms of nicotinic acetylcholine receptor (nAChR) signaling and trafficking in cells.Significance of the studyMost of us are affected by nicotine, not only because of the common use of tobacco products. Nicotine is also included in many popular vegetables of the family Solanaceae, such as tomatoes and peppers. However, these two sources provide the body with radically different doses of nicotine.Strong biological effects of nicotine rely on binding to the nicotinic receptor, which partially mimics the action of the natural hormone acetylcholine. In our study we used the nematode Caenorhabditis elegans as a model organism. General principles of functioning of human cells and C. elegans cells are similar. The worm is, however, a compromise between simplicity and complexity, which may facilitate understanding of the more complex systems, like the human body. Our study revealed in the presence of a low nicotine concentration a different composition of polypeptides in the organism than in the presence of a high nicotine concentration. The rearrangements of protein complexes concern proteins involved e.g. in the course of Alzheimer’s disease, which seems interesting in the context of our aging societies. From the perspective of the development of biological research, the ability to identify the components of large protein complexes can contribute to a better understanding of the functioning of cells.

Publisher

Cold Spring Harbor Laboratory

Reference54 articles.

1. WHO, WHO global report: mortality attributable to tobacco. http://www.who.int/tobacco/publications/surveillance/rep_mortality_attributable/en/ 2012.

2. Is cancer triggered by altered signalling of nicotinic acetylcholine receptors?

3. Cell signaling pathways activated by nicotine;Advances in Cell Biology,2011

4. The nicotinic acetylcholine receptor gene family of the nematode Caenorhabditis elegans: an update on nomenclature

5. Editorial: To channel or not to channel? Functioning of nicotinic acetylcholine receptors in leukocytes

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3