Mapping segmental and sequence variations among laboratory mice using BAC array CGH

Author:

Snijders Antoine M.,Nowak Norma J.,Huey Bing,Fridlyand Jane,Law Sindy,Conroy Jeffrey,Tokuyasu Taku,Demir Kubilay,Chiu Readman,Mao Jian-Hua,Jain Ajay N.,Jones Steven J.M.,Balmain Allan,Pinkel Daniel,Albertson Donna G.

Abstract

We used arrays of 2069 BACs (1303 nonredundant autosomal clones) to map sequence variation among Mus spretus (SPRET/Ei and SPRET/Glasgow) and Mus musculus (C3H/HeJ, BALB/cJ, 129/J, DBA/2J, NIH, FVB/N, and C57BL/6) strains. We identified 80 clones representing 74 autosomal loci of copy number variation (|log2ratio| ≥ 0.4). These variant loci distinguish laboratory strains. By FISH mapping, we determined that 63 BACs mapped to a single site on C57BL/6J chromosomes, while 17 clones mapped to multiple chromosomes (n = 16) or multiple sites on one chromosome (n = 1). We also show that small ratio changes (Δ log2ratio ∼ 0.1) distinguish homozygous and heterozygous regions of the genome in interspecific backcross mice, providing an efficient method for genotyping progeny of backcrosses.

Publisher

Cold Spring Harbor Laboratory

Subject

Genetics (clinical),Genetics

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