Author:
Rakyan Vardhman K.,Down Thomas A.,Thorne Natalie P.,Flicek Paul,Kulesha Eugene,Gräf Stefan,Tomazou Eleni M.,Bäckdahl Liselotte,Johnson Nathan,Herberth Marlis,Howe Kevin L.,Jackson David K.,Miretti Marcos M.,Fiegler Heike,Marioni John C.,Birney Ewan,Hubbard Tim J.P.,Carter Nigel P.,Tavaré Simon,Beck Stephan
Abstract
We report a novel resource (methylation profiles of DNA, or mPod) for human genome-wide tissue-specific DNA methylation profiles. mPod consists of three fully integrated parts, genome-wide DNA methylation reference profiles of 13 normal somatic tissues, placenta, sperm, and an immortalized cell line, a visualization tool that has been integrated with the Ensembl genome browser and a new algorithm for the analysis of immunoprecipitation-based DNA methylation profiles. We demonstrate the utility of our resource by identifying the first comprehensive genome-wide set of tissue-specific differentially methylated regions (tDMRs) that may play a role in cellular identity and the regulation of tissue-specific genome function. We also discuss the implications of our findings with respect to the regulatory potential of regions with varied CpG density, gene expression, transcription factor motifs, gene ontology, and correlation with other epigenetic marks such as histone modifications.
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics(clinical),Genetics
Cited by
320 articles.
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