Author:
Formstecher Etienne,Aresta Sandra,Collura Vincent,Hamburger Alexandre,Meil Alain,Trehin Alexandra,Reverdy Céline,Betin Virginie,Maire Sophie,Brun Christine,Jacq Bernard,Arpin Monique,Bellaiche Yohanns,Bellusci Saverio,Benaroch Philippe,Bornens Michel,Chanet Roland,Chavrier Philippe,Delattre Olivier,Doye Valérie,Fehon Richard,Faye Gérard,Galli Thierry,Girault Jean-Antoine,Goud Bruno,de Gunzburg Jean,Johannes Ludger,Junier Marie-Pierre,Mirouse Vincent,Mukherjee Ashim,Papadopoulo Dora,Perez Franck,Plessis Anne,Rossé Carine,Saule Simon,Stoppa-Lyonnet Dominique,Vincent Alain,White Michael,Legrain Pierre,Wojcik Jérôme,Camonis Jacques,Daviet Laurent
Abstract
The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based technology to screen 102 bait proteins from Drosophila melanogaster, most of them orthologous to human cancer-related and/or signaling proteins, against high-complexity fly cDNA libraries. More than 2300 protein-protein interactions (PPI) were identified, of which 710 are of high confidence. The computation of a reliability score for each protein-protein interaction and the systematic identification of the interacting domain combined with a prediction of structural/functional motifs allow the elaboration of known complexes and the identification of new ones. The full data set can be visualized using a graphical Web interface, the PIMRider (http://pim.hybrigenics.com), and is also accessible in the PSI standard Molecular Interaction data format. Our fly Protein Interaction Map (PIM) is surprisingly different from the one recently proposed by Giot et al. with little overlap between the two data sets. Analysis of the differences in data sets and methods suggests alternative strategies to enhance the accuracy and comprehensiveness of the post-genomic generation of broad-scale protein interaction maps.
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics(clinical),Genetics