Quantitative definition of neurobehavior, vision, hearing and brain volumes in macaques congenitally exposed to Zika virus

Author:

Koenig Michelle R.ORCID,Razo Elaina,Mitzey AnnORCID,Newman Christina M.ORCID,Dudley Dawn M.ORCID,Breitbach Meghan E.ORCID,Semler Matthew R.,Stewart Laurel M.,Weiler Andrea M.,Rybarczyk Sierra,Bach Kathryn M.,Mohns Mariel S.,Simmons Heather A.,Mejia AndresORCID,Fritsch Michael,Dennis Maria,Teixeira Leandro B. C.ORCID,Schotzko Michele L.,Nork T. Michael,Rasmussen Carol A.ORCID,Katz Alex,Nair Veena,Hou Jiancheng,Hartman Amy,Hoeve James VerORCID,Kim Charlene,Schneider Mary L.,Ausderau Karla,Kohn Sarah,Jaeger Anna S.,Aliota Matthew T.ORCID,Hayes Jennifer M.ORCID,Schultz-Darken NancyORCID,Eickhoff Jens,Antony Kathleen M.ORCID,Noguchi Kevin,Zeng XiankunORCID,Permar Sallie,Prabhakaran Vivek,Capuano Saverio,Friedrich Thomas C.ORCID,Golos Thaddeus G.,O’Connor David H.ORCID,Mohr Emma L.ORCID

Abstract

AbstractCongenital Zika virus (ZIKV) exposure results in a spectrum of disease ranging from severe birth defects to delayed onset neurodevelopmental deficits. ZIKV-related neuropathogenesis, predictors of birth defects, and neurodevelopmental deficits are not well defined in people. Here we assess the methodological and statistical feasibility of a congenital ZIKV exposure macaque model for identifying infant neurobehavior and brain abnormalities that may underlie neurodevelopmental deficits. We inoculated five pregnant macaques with ZIKV and mock-inoculated one macaque in the first trimester. Following birth, growth, ocular structure/function, brain structure, hearing, histopathology, and neurobehavior were quantitatively assessed during the first week of life. We identified the typical pregnancy outcomes of congenital ZIKV infection, with fetal demise and placental abnormalities. We estimated sample sizes needed to define differences between groups and demonstrated that future studies quantifying brain region volumes, retinal structure, hearing, and visual pathway function require a sample size of 14 animals per group (14 ZIKV, 14 control) to detect statistically significant differences in at least half of the infant exam parameters. Establishing the parameters for future studies of neurodevelopmental outcomes following congenital ZIKV exposure in macaques is essential for robust and rigorous experimental design.

Publisher

Cold Spring Harbor Laboratory

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3