Author:
Bascos Neil Andrew D.,Mirano-Bascos Denise,Saloma Cynthia P.
Abstract
ABSTRACTA SARS-CoV-2 lineage designated as P.3 with multiple signature mutations in the Spike protein region was recently reported with cases from the Central Visayas Region of the Philippines. Whole genome sequencing revealed that the 33 samples under this lineage all contain the E484K, N501Y, and P681H Spike mutations previously found in variants of concern (VOC) such as the B.1.351, the P.1 and B.1.1.7 variants first reported in South Africa, Brazil, and the United Kingdom, respectively. The possible implications of the mutations found in the Spike protein of P.3 were analyzed for their potential effects on structure, stability, and molecular surface character. The analysis suggests that these mutations could significantly impact the possible interactions of the Spike protein with the ACE2 receptor and neutralizing antibodies, and warrants further clinical investigation. Some of the mutations affecting the N and C terminal domains may have effects on Spike monomer and trimer stability. This report provides insights on relevant targets for the design of future diagnostics, therapeutics and vaccines against the evolving SARS-CoV-2 variants in the Philippines.
Publisher
Cold Spring Harbor Laboratory
Reference36 articles.
1. Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability
2. Cheng, M. H. , Krieger, J. M. , Kaynak, B. , Arditi, M. A. , & Bahar, I. (2021). Impact of South African 501. V2 variant on SARS-CoV-2 spike infectivity and neutralization: A structure-based computational assessment. bioRxiv 2021.01.10.426143; doi: https://doi.org/10.1101/2021.01.10.426143
3. The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade
4. COVID-19 Tracker | Department of Health website (doh.gov.ph) https://doh.gov.ph/covid19tracker
5. Garry, R.F. , Andersen, K.G. ET AL (2020). Spike protein mutations in novel SARS-CoV-2 ‘variants of concern’ commonly occur in or near indels. image - virological.org
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献