Antibiotic Resistance in Bacillus-based Biopesticide Products

Abstract

AbstractThe crisis of antibiotic resistant bacterial infections is one of the most pressing public health issues. Common agricultural practices have been implicated in the generation of antibiotic resistant bacteria. Biopesticides, live bacteria used for pest control, are non-pathogenic and considered safe for consumption. Application of bacteria-based pesticides to crops in high concentrations raises the possibility of unintentional contributions to the movement and generation of antibiotic resistance genes in the environment. However, the presence of clinically relevant antibiotic resistance genes and their resistance phenotypes are currently unknown. Here we use a combination of multiple bioinformatic and microbiological techniques to define resistomes of widely used biopesticides and determine how the presence of suspected antibiotic resistance genes translates to observable resistance phenotypes in several biopesticide products. Our results demonstrate that biopesticide products are reservoirs of clinically relevant antibiotic resistance genes and bear resistance to multiple drug classes.ImportanceThis is the first study to specifically address antibiotic resistance in widely distributed bacterial strains used as commercial biopesticides. Safety assessments of commercial live bacterial biopesticide products do not include antibiotic resistance phenotype identification. We identify antibiotic resistance genes in all live bacterial strains examined, and resistant phenotypes in all strains tested for antibiotic susceptibility. This work demonstrates that biopesticides potentially play a critical role as reservoirs and vectors of antibiotic resistance in the broader environmental resistome that is to date, unstudied.