Author:
Kim Kyoung Mi,Noh Ji Heon,Bodogai Monica,Martindale Jennifer L.,Yang Xiaoling,Indig Fred E.,Basu Sandip K.,Ohnuma Kei,Morimoto Chikao,Johnson Peter F.,Biragyn Arya,Abdelmohsen Kotb,Gorospe Myriam
Abstract
Senescent cell accumulation in aging tissues is linked to age-associated diseases and declining function, prompting efforts to eliminate them. Mass spectrometry analysis revealed that DPP4 (dipeptidyl peptidase 4) was selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. Importantly, the differential presence of DPP4 allowed flow cytometry-mediated isolation of senescent cells using anti-DPP4 antibodies. Moreover, antibody-dependent cell-mediated cytotoxicity (ADCC) assays revealed that the cell surface DPP4 preferentially sensitized senescent, but not dividing, fibroblasts to cytotoxicity by natural killer cells. In sum, the selective expression of DPP4 on the surface of senescent cells enables their preferential elimination.
Funder
National Institute on Aging
National Cancer Institute
National Institutes of Health
Japan Society for the Promotion of Science
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Cited by
198 articles.
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