Mutations in the U2 snRNA geneRNU2-2Pcause a severe neurodevelopmental disorder with prominent epilepsy

Abstract

The major spliceosome comprises the five snRNAs U1, U2, U4, U5 and U6. We recently showed that mutations inRNU4-2, which encodes U4 snRNA, cause one of the most prevalent monogenic neurodevelopmental disorders. Here, we report that recurrent germline mutations inRNU2-2P, a 191bp gene encoding U2 snRNA, are responsible for a related disorder. By genetic association, we implicated recurrentde novosingle nucleotide mutations at nucleotide positions 4 and 35 ofRNU2-2Pamong nine cases. We replicated this finding in six additional cases, bringing the total to 15. The disorder is characterized by intellectual disability, neurodevelopmental delay, autistic behavior, microcephaly, hypotonia, epilepsy and hyperventilation. All cases display a severe and complex seizure phenotype. Our findings cement the role of major spliceosomal snRNAs in the etiologies of neurodevelopmental disorders.