Nanomotion technology for testing azithromycin susceptibility ofSalmonella enterica

Author:

Hinnu MariliisORCID,Mets Toomas,Kerkez Ivana,Putrinš Marta,Kaldalu NiiloORCID,Cathomen Gino,Verge Marta Pla,Cichocka Danuta,Sturm AlexanderORCID,Tenson TanelORCID,

Abstract

ABSTRACTAzithromycin is used to treat invasive salmonellosis, despite conflicting effective concentrationsin vitroandin vivo. Resistance ofSalmonella entericato azithromycin is increasing. We demonstrate that nanomotion technology can be used for rapid phenotypic testing ofSalmonella’s susceptibility to azithromycin. Nanomotion changes under various culture conditions correlated with susceptibility measured by MIC determination, CFU counting, and fluorescent reporter-based estimates of intrabacterial azithromycin accumulation.

Publisher

Cold Spring Harbor Laboratory

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