Abstract
AbstractUnderstanding spatial gene expression and regulation is key to uncovering developmental and physiological processes, during homeostasis and disease. Numerous techniques exist to gain gene expression and regulation information, but very few utilise intuitive true-to-life three-dimensional representations to analyze and visualize results. Here we combined spatial transcriptomics with 3D modelling to represent and interrogate, transcriptome-wide, three-dimensional gene expression and location in the mouse adult heart. Our study has unveiled specific subsets of genes that display complex spatial expression in organ sub-compartments. Also, we created a web-based user interface for spatial transcriptome analysis and visualization. The application may be accessed from http://3d-cardiomics.erc.monash.edu/.
Publisher
Cold Spring Harbor Laboratory
Reference60 articles.
1. Developmental Origin, Growth, and Three-Dimensional Architecture of the Atrioventricular Conduction Axis of the Mouse Heart
2. A novel Myosin essential light chain mutation causes hypertrophic cardiomyopathy with late onset and low expressivity;Biochemistry research international,2012
3. The anatomy of the cardiac conduction system
4. Anders, S. , Pyl, P.T. & Huber, W. , 2010. HTSeq: Analysing high-throughput sequencing data with Python.
5. Andrews, S. & Others, 2010. FastQC: a quality control tool for high throughput sequence data.
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献