Abstract
AbstractCLARITY is a tissue clearing method, which enables immunostaining and imaging of large volumes for 3Dreconstruction. The method was initially time-consuming, expensive and relied on electrophoresis to remove lipids to make the tissue transparent. Since then several improvements and simplifications have emerged, such as passive clearing (PACT) and methods to improve tissue staining. Here, we review advances and compare current applications with the aim of highlighting needed improvements as well as aiding selection of the specific protocol for use in future investigations.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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1. Histology and Embryology 4.0;Perspectives on Artificial Intelligence in Times of Turbulence;2023-11-24