Antioxidant, Anti-inflammatory and Anticancer activities of Methanolic Extract of Thottea siliquosa: An In Vitro study

Abstract

AbstractBackground and AimOxidative stress and inflammation are the predominant cause of chronic diseases including cancers, thereby making it as an essential target for preventing these disorders. Various natural products and plants have been shown to prevent the process of free radical induced damages. The present study evaluated the biological properties of Thottea siliquosa, belonging to the family Aristolochiaceae, which is a traditionally used Ayurvedic plant.Experimental ProcedureAntioxidant assays carried out were DPPH, FRAP, hydrogen peroxide scavenging and hemolysis inhibition assay; nitric oxide and lipoxygenase inhibition assays were used for anti-inflammatory studies. Anticancer activity was done using human endometrial and breast cancer cells by MTT assay.Results and ConclusionThe results of the present study showed antioxidant properties for the methanolic crude extract of T. siliquosa as DPPH radical scavenging (110.40± 4.5μg/mL), FRAP capacity (41.1± 6.2), peroxide scavenging (233.4± 14.2 μg/mL) etc. In addition, anti-inflammatory properties are also evident in terms of nitric oxide radical scavenging (28.76± 3.9 μg/mL) and lipoxygenase inhibition (39.2± 3.2 μg/mL) assays. Further, the anticancer activity of these extract has been identified against human endometrial and breast cancer cells. Further studies together with a bioassay guided fractionation may identify possible bioactive compounds.HighlightsThottea siliquosa is a traditional Ayurvedic medicineT. siliquosa exhibits significant anti-inflammatory and anticancer propertiesPolyphenolic compounds may be responsible for the bioactivities of T. siliquosaT. siliquosa may be a source for novel bioactive components