Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples

Author:

Eis-Hübinger Anna M.,Hönemann Mario,Wenzel Jürgen J.,Berger Annemarie,Widra Marek,Schmidt Barbara,Aldabbagh Souhaib,Marx Benjamin,Streeck Hendrik,Ciesek Sandra,Liebert Uwe. G.,Huzly Daniela,Hengel Hartmut,Panning Marcus

Abstract

AbstractWe report a laboratory-based surveillance for SARS-CoV-2 using minipools of respiratory samples submitted for routine diagnostics. We tested a total of 70 minipools resembling 700 samples shortly before the upsurge of cases in Germany. We identified one SARS-CoV-2 positive patient. Our approach proved its concept, is easily adaptable and resource-saving.

Publisher

Cold Spring Harbor Laboratory

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