Potassium Glutamate and Glycine Betaine Induce Self-Assembly of Sliding Clamps into Higher Order Oligomers

Abstract

ABSTRACTSliding clamps are oligomeric ring-shaped proteins that increase the efficiency of DNA replication. The stability of theEscherichia coliβ-clamp, a homodimer, is particularly remarkable. The dissociation equilibrium constant of β is of the order of 10 pM in buffers of moderate ionic strength. Coulombic electrostatic interactions have been shown to contribute to this remarkable stability. Increasing NaCl concentration in the assay buffer results in decreased dimer stability and faster subunit dissociation kinetics in a way consistent with simple charge-screening models. Here, we examine non-Coulombic ionic effects on the oligomerization properties of sliding clamps. Replacing NaCl by KGlu, the primary cytoplasmic salt inE. coli, results in the formation of assemblies that involve two or more rings stacked face-to-face. Results can be quantitatively explained on the basis of unfavorable interactions between KGlu and the functional groups on the protein surface, which drive biomolecular processes that bury exposed surface. Similar results were obtained with theS. cerevisiaePCNA sliding clamp, suggesting that KGlu effects are not specific to β. Clamp association is also promoted by glycine betaine, a zwitterionic compound that accumulates intracellularly whenE. coliis exposed to high concentrations of extracellular solute. Possible biological implications are discussed.