Proteome comparison between natural desiccation-tolerant plants and drought-protected Caspicum annuum plants by Microbacterium sp. 3J1

Abstract

ABSTRACTDesiccation-tolerant plants are able to survive for extended periods of time in the absence of water. The molecular understanding of the mechanisms used by these plants to resist droughts can be of great value for the improvement of tolerance of sensitive plants with agricultural interest. This understanding is especially relevant in an environment that tends to increase the number and intensity of droughts. The combination of certain microorganisms with drought-sensitive plants can improve their tolerance to water scarcity. One of these bacteria is Microbacterium sp. 3J1, an actinobacteria able to protect pepper plants from drought. In this study we describe the proteome of the interaction of Microbacterium sp. 3J1 with pepper plants during droughts. We also compare this proteome with the proteome found in desiccation-tolerant plants. In addition, we studied the proteome of Microbacterium sp. 3J1 subjected to drought to analyze its contribution to the plant-microbe interaction. We describe those mechanisms shared by desiccation-tolerant plants and sensitive plants protected by microorganisms focusing on protection against oxidative stress, and production of compatible solutes, plant hormones, and other more specific proteins.IMPORTANCEMaintaining agricultural production under greater number and intensity of droughts is one of the main global challenges. Some plants can survive in the absence of water for extended periods of time. The molecular understanding of the mechanisms used by these plants to resist droughts is of great interest for the development of new strategies to face this challenge. Some microorganisms protect sensitive plants to some extent from droughts. Microbacterium sp. 3J1, is an actinobacteria able to protect pepper plants from drought. In this study we describe the different protein profile under drought used by the plant during the interaction with the microorganism and compare it with the one presented by desiccation-tolerant plants and with the one presented by Microbacterium sp. 3J1 to analyze its contribution to the plant-microbe interaction. We describe those mechanisms focusing on protection against oxidative stress, and production of compatible solutes, plant-hormones, and other more specific proteins.