Author:
Wang Jianpeng,Ren Keqin,Pérez Javier,Silva Alcino J.,Peña de Ortiz Sandra
Abstract
We examined the hypothesis that processes related to DNA recombination and
repair are involved in learning and memory. Rats received
intracerebroventricular (icv) infusions of the antimetabolite
1-beta-D-arabinofuranosylcytosine triphosphate (ara-CTP) or its precursor
cytosine arabinoside (ara-C) 30 min prior to conditioned taste aversion (CTA)
training. Both ara-CTP and ara-C caused significant impairments in long-term
memory (LTM) of CTA. Control experiments indicate that the effect of ara-CTP
on CTA memory is related to interference with learning. Furthermore, as it was
previously demonstrated for the protein synthesis inhibitor anisomycin,
ara-CTP had no effect on CTA memory when it was injected 1 h after training.
Importantly, although both ara-CTP and anisomycin significantly blocked LTM in
the task, short-term memory (STM) measured 1 h after training was not affected
by either of the drugs. Finally, ara-CTP had no effect on in vitro
transcription, but it did effectively block nonhomologous DNA end joining
(NHEJ) activity of brain protein extracts. We suggest that DNA ligase-mediated
DNA recombination and repair processes are necessary for the expression of LTM
in the brain.
Publisher
Cold Spring Harbor Laboratory
Subject
Cellular and Molecular Neuroscience,Cognitive Neuroscience,Neuropsychology and Physiological Psychology
Cited by
18 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献