Abstract
The bacterial transposon Tn7 is capable of high-frequency transposition to a specific site in the Escherichia coli chromosome, attTn7, and of low-frequency transposition to sites other than attTn7. Using an in vitro insertional mutagenesis procedure, we have identified and characterized five tns (Tn seven) genes that are essential for Tn7 transposition. Three of these genes, tnsA, tnsB, and tnsC, are required, but are not sufficient, for all Tn7 transposition events. In addition, tnsD is specifically required for transposition to attTn7, whereas tnsE is specifically required for transposition to other sites. Thus, Tn7 is an elaborate transposon that encodes two distinct but overlapping transposition pathways.
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Reference49 articles.
1. DNA sequence of theE. coli gyrB gene: application of a new sequencing strategy
2. Map of plasmid RP4 derived by insertion of transposon C
3. Conjugal transfer system of plasmid RP4: Analysis by transposon 7 insertion.;J. Bacteriol.,1978
4. Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons.;J. Bacteriol.,1976
5. Berg, D.E. 1977. Insertion and excision of the transposable kanamycin resistance determinant Tn5. In DNA insertion elements, plasmids, and episomes (ed. A.I. Bukhari, J.A. Shapiro, and S.L. Adhya), pp. 205–212. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
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