Author:
Yang X J,Hart C M,Grayhack E J,Roberts J W
Abstract
The gene Q protein of phage lambda is a transcription antiterminator that modifies RNA polymerase near the phage late gene promoter and thereby causes antitermination at distant sites. To define the site of action of Q protein, we have reconstructed the regulatory system on plasmids that allow the intracellular concentration of Q protein to be regulated, and that allow the effect of Q protein on transcription from variant promoter segments to be measured in vivo and in vitro. We show that DNA sequences essential for Q protein-mediated antitermination span the RNA start site, but do not extend beyond nucleotide 18 of the late RNA coding region. We also show that the modification that permits antitermination persists while RNA polymerase passes at least two terminators in vivo and in vitro.
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Reference20 articles.
1. A block to elongation is largely responsible for decreased transcription of c-myc in differentiated HL60 cells
2. The cis specificity of the Q gene product of bacteriophage lambda.;Mol. Gen. Genet.,1982
3. Daniels, D., J. Schroeder, W. Szybalski, F. Sanger, A. Coulson, G. Hong, D. Hill, G. Petersen, and F. Blattner. 1983. Complete annotated lambda sequence. In Lambda II (ed. R. Hendrix, J. Roberts, F. Stahl, and R. Weisberg), pp. 519â676. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
4. Specificity of the bacteriophage lambda N gene product (pN): Nut sequences are necessary and sufficient for antitermination by pN
5. On the nature of cis-acting regulatory proteins and genetic organization in bacteriophage: The example of gene Q of bacteriophage lambda.;Genetics,1976
Cited by
51 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献