Author:
Yahiro Takaki,Bayless-Edwards Landon,Jones James A.,Ma Lei,Qin Maozhen,Mao Tianyi,Zhong Haining
Abstract
AbstractWe report a genetically encoded fluorescence lifetime sensor for protein kinase C (PKC) activity, named CKAR3, based on Förster resonance energy transfer. CKAR3 exhibits a 10-fold increased dynamic range compared to its parental sensors and enablesin vivoimaging of PKC activity during animal behavior. Our results reveal robust PKC activity in a sparse neuronal subset in the motor cortex during locomotion, in part mediated by muscarinic acetylcholine receptors.
Publisher
Cold Spring Harbor Laboratory