Chronic wounds and adaptivePseudomonas aeruginosa: A phenotypic and genotypic characterization

Abstract

AbstractPhenotypic and genetic diversity is found in varying prevalence in clinical populations where beneficial adaptations enable the bacteria to avoid recognition and eradication by the host immune system. This study aimed to investigate the presence ofPseudomonas aeruginosain chronic venous leg ulcers wounds over an 8-week time course. This was performed using genomic and phenotypic approaches to understand the survival and persistence ofPseudomonasstrains. The findings of this study show that the two patients were colonized with a recurringP. aeruginosagenotype with only minor phenotypic differences and few SNP differences, suggesting that thePseudomonasisolates present in the wound can survive and proliferate in the host’s hostile environment. The results provided from this study will allow us to understand clinically important traits inP. aeruginosaand provide insight into the dynamics and adaptations (or the lack thereof) in chronic wounds.HighlightsEach patient is colonised overtime with a unique MLSTThe total number of detected SNPs was 18 and 20 within each patient, respectivelyOne MLST belonged to the sequence type ST132, often observed in clinical isolates, whereas the other belonged to ST3244, previously seen inenvironmental isolates