SID-2 localises to extracellular vesicles in parasitic nematodes and does not function in environmental RNAi

Abstract

ABSTRACTIn the free-living nematodeCaenorhabditis elegansthe transmembrane protein SID-2 imports double-stranded RNA into intestinal cells to trigger systemic RNA interference (RNAi), allowing organisms to sense and respond to environmental cues such as the presence of pathogens. This process, known as environmental RNAi, has not been observed in the most closely related parasites that are also within clade V. Previous sequence-based searches failed to identifysid-2orthologs in available clade V parasite genomes. In this study we identifiedsid-2orthologues in these parasites using genome synteny and protein structure-based comparison, following identification of a SID-2 orthologue in extracellular vesicles from the murine intestinal parasitic nematodeHeligmosomoides bakeri. Expression of GFP-taggedH. bakeriSID-2 inC. elegansshowed comparable localisation to the intestinal apical membrane as seen for GFP-taggedC. elegansSID-2 and further showed mobility in intestinal cells in vesicle-like structures. We tested the capacity ofH. bakeriSID-2 to functionally complement environmental RNAi in aC. elegansSID-2 null mutant and show thatH. bakeriSID-2 does not rescue the phenotype in this context. Therefore, our work identifies SID-2 as a highly abundant nematode EV protein whose ancestral function may be unrelated to environmental RNA and rather highlights an association with extracellular vesicle-mediated communication in free-living and parasitic nematodes.