Abstract
AbstractCtenophores are an attractive lineage for studying animal evolution due to their early divergence from other metazoans. Among Ctenophora,Mnemiopsis leidyiis a model system for developmental, cellular, molecular genetic, and evolutionary studies. Until recently, many of these studies were conducted on wild-caught animals, limiting access to researchers on the coast. Here we present significant advancements towards culturingM. leidyiin laboratories without coastal access, enabling its wider use as an experimental and genetic model system. We detail updated feeding regimes that take advantage of co-culturingBrachionusrotifers withApocyclopscopepods, and quantify the reproductive output of ourM. leidyilab strain on this diet. Our updated feeding regime maintains reproductive fitness comparable to wild-caught individuals. Importantly, we have eliminated the logistical complexities and costs of regularly feeding live larval fish toM. leidyi. Our updated protocols make it feasible to maintain continuous ctenophore cultures independent of access to both coastal populations of wildM. leidyiand larval fish culturing facilities.
Publisher
Cold Spring Harbor Laboratory