Effects of n-Hexane and ethanol extract ofMilicia excelsastone on pain perception in Sodium Monoiodoacetate-induced Osteoarthritic Female Wistar Rats

Abstract

ABSTRACTAIMThis research was undertaken to evaluate the effect of n-Hexane and ethanol extract ofMilicia excelsastone on pain perception in Sodium Monoiodoacetate-induced Osteoarthritic Female Wistar Rats. And the most active ingredient inMilicia excelsastone is calcium carbonate.METHODFor this study, fifty (50) female Wistar rats divided into five groups were used, these include: Positive control (normal health rats), Negative control (osteoarthritic rats with no treatment), Reference (osteoathritic rats treated with arthocare), Test (1) (osteoarthritic rats treated with n-Hexane extract ofmilicia excelsastone), Test (2) (osteoarthritic rats treated with ethanol extract ofmilicia excelsastone). Von Frey Hair Filament was used to access pain perception across the groups. And the knee circumference was taken pre-induction, induction, and post-induction.RESULTSThe result of this experimental research showed that there was significant (p<0.05) decrease both in the cartilage and serum level of biomarkers such as Prostanglandin E2, Tumour Necrotic factor alpha, Vascular endothelial growth factor, and Cartillage oligomeric metalloprotein. There was significance (p<0.05) decrease in the knee oedema. Also, the pain threshold increased in both n-Hexane and ethanol groups.CONCLUSIONIt is therefore concluded that n-Hexane and ethanol extracts ofMilicia excelsahave significant effect in reducing pain perception in monosodium iodoacetate-induced osteoarthritic female Wistar rats.