Molecular Epidemiology of DiarrhoeagenicEscherichia coliin Africa: A Systematic Review and Meta-Analysis

Abstract

AbstractIntroductionDiarrhoeagenicEscherichia coli(DEC) persistently challenges public health in Africa, contributing substantially to the diarrhoeal disease burden. This systematic review and meta-analysis illuminate the distribution and antimicrobial resistance (AMR) patterns of DEC pathotypes across the continent.MethodsThe review selectively focused on studies reporting prevalence and/or AMR of human-derived DEC pathotypes from African nations, excluding data from extra-intestinal, animal, and environmental sources, and studies focused on drug and mechanism experiments. Employing a robust search strategy, pertinent studies were retrieved from SCOPUS, PubMed, and EBSCOhost, processed with Covidence, and screened in alignment with PRISMA guidelines.ResultsThe reviewed studies were predominantly hospital-based (80%) and paediatric-focused (91%), with a meagre 4.4% documenting DEC outbreaks. Seven DEC pathotypes were discerned, with EnteroaggregativeE. coli(EAEC) being notably prevalent (43%, 95% CI: 30% – 55%) and EnteroinvasiveE. coli(EIEC) least prevalent (24%, 95%CI: 17% – 32%). Identified non-susceptibilities were noted against essential antibiotics, including ciprofloxacin, ceftriaxone, and ampicillin, while instances of carbapenem and Extended-Spectrum Beta-Lactamase (ESBL) resistance were scarce.ConclusionDespite sporadic data on DEC prevalence and AMR in Africa, particularly in community settings, a palpable gap remains in real-time outbreak surveillance and comprehensive data documentation. Augmenting surveillance and embracing advancements in molecular/genomic characterisation techniques are crucial to precisely discerning the actual impact and resistance continuum of DEC in Africa.