Abstract
AbstractBackgroundThe widespread use of conventional pesticides to control plant fungal and bacterial pathogens poses significant risks to human health and the environment, and there is an urgent need for safer and more sustainable alternatives in agricultural management. Studies have shown that plant extracts can be effective in controlling plant diseases either by directly targeting the pathogens or by reinforcing the host plant’s own defenses. Here, we examined the potential of ethanolic extracts from forest tree species eastern hemlock, English oak, eastern red cedar and red pine for their antibacterial activity againstPseudomonas syringaepv.tomato(Pst) strain DC3000 and the ability of these forestry by-products to trigger effective defense responses in the model plantArabidopsis thaliana.ResultsThe four tree extracts exhibited direct toxic effects againstPstDC3000, as notably observed for the English oak extract inhibiting bacterial growth and showing bactericidal activity at relatively low concentrations. Using an Arabidopsis line expressing reporter protein ß-glucuronidase under the control of a salicylic acid-inducible pathogenesis-related protein gene promoter, the extracts were shown also to induce defense-related genes expression in leaf tissue. RT-qPCR assays with DNA primers for different gene markers further confirmed this conclusion and highlighted gene-inducing effects for the tree extracts triggering, at different rates, the expression of salicylic acid- and oxidative stress-responsive genes. The extracts direct antibacterial effects, combined with their defense gene-inducing effectsin planta, resulted in a strong host plant-protecting effect againstPstDC3000 associated with bacterial growth rates reduced by ∼75 to 98% seven days post-infection, depending on the extract.ConclusionsThese findings show the effectiveness of tree extracts as eventual plant protectants against the plant bacterial pathogenPst. In a broader perspective, they suggest the potential of these forestry by-products as a source of bioactive compounds useful in plant protection and as a sustainable, eco-friendly alternative to conventional synthetic pesticides for the management of economically important plant pathogens.
Publisher
Cold Spring Harbor Laboratory
Reference77 articles.
1. The persistent threat of emerging plant disease pandemics to global food security
2. Roller W. Chemical approaches to managing plant pathogens. In: Handbook of Pest Management (1st Ed.). Ruberson JR (ed.). Boca Raton, FL , USA. CRC Press; 1999. p. 359–398.
3. Haq IU , Ijaz S (eds.). Plant disease management strategies for sustainable agriculture through traditional and modern approaches. (1st Ed.) Cham, Switzerland Springer Nature Switzerland AG. 2020. XIII, 339.
4. Pesticides in agriculture and environment: Impacts on human health,2019
5. Agrochemicals, environment, and human health;Annu Rev Environ Res,2022