The effects of genome size on cell size and the functional composition and morphology of leaves: a case study inRhododendron(Ericaceae)

Author:

Dastpak Arezoo,Williams Monica,Perkins Sally,Perkins John A.,Horn Charles,Thompson Patrick,Ryan Connor,Medeiros Juliana,An Yi-Dong,Jiang Guo-Feng,Simonin Kevin A.,Roddy Adam B.ORCID

Abstract

AbstractBackground and AimsDespite the predominance of scaling photosynthetic metabolism by two-dimensional leaf surface area, leaves are three-dimensional structures composed of multiple tissues that directly and indirectly influence photosynthetic metabolism. The structure of leaf surfaces for CO2diffusion and light transmission and the internal volume of tissues that process energy and matter work together to control rates of resource acquisition and turnover. Here we investigate the influence of cell size and packing density on resource acquisition as measured by surface conductance to CO2and water vapor and on resource turnover as measured by leaf water turnover time.MethodsWe sampled wild and cultivated congeneric species in the genusRhododendron(Ericaceae) and measured genome size, anatomical traits related to cell sizes and packing densities, and morphological traits related to water content and dry mass allocation.ResultsAmongRhododendron, anatomical traits related to cell size and morphological traits related to water content and dry mass investment varied largely orthogonally to each other, allowing for many combinations of leaf traits to exist. However, there was a strong, negative relationship between the leaf water residence time (τ) and the maximum leaf surface conductance per leaf volume (gmax,vol), both of which are influenced by cell size and cell packing densities.ConclusionsDespite leaf function being controlled by many potential combinations of leaf cell- and tissue-level traits, cell size has a pervasive effect on leaf function. Small cells allow for higher diffusion of CO2and water vapor per unit leaf volume (gmax,vol) even at constant leaf thickness, but small cells also result in shorter leaf water residence times (τ). The strong tradeoff betweengmax,voland (τ) illuminates how genome size-cell size allometry influences the fast-slow continuum of plant carbon and water economy.

Publisher

Cold Spring Harbor Laboratory

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