Impaired Mitochondrial Calcium Homeostasis Induces Failed Remodeling of the Spiral Artery in Preeclampsia

Author:

Lu Xiyuan,Wang Yifan,Zou Zhiguo,Wang Chuan,Feng Xueqing,Geng Na,Wang Yuehong,Xu Zhice,Pu Jun

Abstract

ABSTRACTRATIONALEPreeclampsia (PE) is a hypertensive disorder that occurs during pregnancy and is characterized by failed spiral artery remodeling. Calcium deficiency in women has been strongly linked to an increased risk of developing PE. Mitochondrial calcium ([Ca2+]m) homeostasis is essential to regulate vascular smooth muscle cell (VSMC) function. However, the role of [Ca2+]min PE development remains largely unknown.METHODSTo investigate this, we measured vessel tone on isolated human spiral arteries obtained from normotensive and hypertensive pregnant women. The PE rat model was induced with L-NAME treatment.RESULTSOur initial findings revealed compromised vessel function in spiral arteries derived from PE patients, as evidenced by diminished vasoconstriction and vasodilation responses to angiotensin II and sodium nitroprusside, respectively. Moreover, the spiral artery VSMCs from PE patients exhibited phenotypic transformation and proliferation associated with imbalanced [Ca2+]mhomeostasis. Subsequent in vitro experiments employing gain- and loss-of-function approaches demonstrated that mitochondrial Na+/Ca2+exchanger (mtNCLX) played a role in promoting VSMCs phenotypic switching and impaired mitochondrial functions. Furthermore, the utilization of the pharmacological inhibitor CGP37157 to block mtNCLX revealed a significant attenuation of VSMC phenotypic switching and restoration of normal mitochondrial function in both the spiral arteries of PE patients and a PE rat model.CONCLUSIONSThis study provides comprehensive evidence supporting the significance of [Ca2+]min VSMCs of spiral arteries, shedding light on its association with VSMC phenotypic switching and the compromised remodeling of spiral arteries in PE. The findings suggest that targeting the mtNCLX holds promise as a novel therapeutic approach for the management of PE.GRAPHIC ABSTRACTGraphic abstract is available for this article.

Publisher

Cold Spring Harbor Laboratory

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3