Author:
Kose Cansu,Sancar Aziz,Jiang Yuchao
Abstract
ABSTRACTWe have adapted the eXcision Repair-sequencing (XR-seq) method to generate single-nucleotide resolution dynamic repair maps of UV-induced cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts in theCaenorhabditis elegans (C. elegans)genome. We focus on theC. elegansortholog of the human XPC-deficient strain (xpc-1) and its exclusive use of transcription-coupled repair. We provide evidence demonstrating the utility ofxpc-1XR-seq as a remarkable tool for detecting nascent transcription and identifying new transcripts. The integration of epigenetic markers, chromatin states, enhancer RNA and long intergenic non-coding RNA annotations supports the robust detection of intergenic nascent transcription by XR-seq. Overall, our results provide a comprehensive view of the transcription-coupled repair landscape inC. elegans, highlighting its potential contributions to our understanding of DNA repair mechanisms and non-coding RNA biology.
Publisher
Cold Spring Harbor Laboratory
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