The Synaptic Architecture of Layer 5 Thick Tufted Excitatory Neurons in the Visual Cortex of Mice-Reference-Cited by-同舟云学术

The Synaptic Architecture of Layer 5 Thick Tufted Excitatory Neurons in the Visual Cortex of Mice

Published:2023-10-20 Issue: Volume: Page:
ISSN:
Container-title:
language:
Short-container-title:

Author:

Bodor Agnes L.^ORCID,Schneider-Mizell Casey M^ORCID,Zhang Chi^ORCID,Elabbady Leila^ORCID,Mallen Alex,Bergeson Andi,Brittain Derrick^ORCID,Buchanan JoAnn^ORCID,Bumbarger Daniel J.,Dalley Rachel^ORCID,Gamlin Clare^ORCID,Joyce Emily^ORCID,Kapner Daniel,Kinn Sam^ORCID,Mahalingam Gayathri^ORCID,Seshamani Sharmishtaa^ORCID,Suckow Shelby,Takeno Marc^ORCID,Torres Russel^ORCID,Yin Wenjing^ORCID,Bae J. Alexander^ORCID,Castro Manuel A.,Dorkenwald Sven^ORCID,Halageri Akhilesh^ORCID,Jia Zhen,Jordan Chris,Kemnitz Nico^ORCID,Lee Kisuk^ORCID,Li Kai,Lu Ran^ORCID,Macrina Thomas^ORCID,Mitchell Eric,Mondal Shanka Subhra,Mu Shang,Nehoran Barak^ORCID,Popovych Sergiy,Silversmith William^ORCID,Turner Nicholas L.^ORCID,Yu Szi-chieh^ORCID,Wong William,Wu Jingpeng^ORCID,Celii Brendan,Campagnola Luke,Seeman Stephanie C,Jarsky Tim,Ren Naixin,Arkhipov Anton,Reimer Jacob,Seung H Sebastian^ORCID,Reid R. Clay^ORCID,Collman Forrest^ORCID,Maçarico da Costa Nuno^ORCID,

Abstract

SummaryThe neocortex is one of the most critical structures that makes us human, and it is involved in a variety of cognitive functions from perception to sensory integration and motor control. Composed of repeated modules, or microcircuits, the neocortex relies on distinct cell types as its fundamental building blocks. Despite significant progress in characterizing these cell types1–5, an understanding of the complete synaptic partners associated with individual excitatory cell types remain elusive.Here, we investigate the connectivity of arguably the most well recognized and studied excitatory neuron in the neocortex: the thick tufted layer 5 pyramidal cell6–10also known as extra telencephalic (ET)11neurons. Although the synaptic interactions of ET neurons have been extensively explored, a comprehensive characterization of their local connectivity remains lacking. To address this knowledge gap, we leveraged a 1 mm3electron microscopic (EM) dataset.We found that ET neurons primarily establish connections with inhibitory cells in their immediate vicinity. However, when they extend their axons to other cortical regions, they tend to connect more with excitatory cells. We also find that the inhibitory cells targeted by ET neurons are a specific group of cell types, and they preferentially inhibit ET cells. Finally, we observed that the most common excitatory targets of ET neurons are layer 5 IT neurons and layer 6 pyramidal cells, whereas synapses with other ET neurons are not as common.These findings challenge current views of the connectivity of ET neurons and suggest a circuit design that involves local competition among ET neurons and collaboration with other types of excitatory cells. Our results also highlight a specific circuit pattern where a subclass of excitatory cells forms a network with specific inhibitory cell types, offering a framework for exploring the connectivity of other types of excitatory cells.

Publisher

Cold Spring Harbor Laboratory

Reference113 articles.

1. Form, function and intracortical projections of spiny neurones in the striate visual cortex of the cat.

2. Objective Morphological Classification of Neocortical Pyramidal Cells

3. Computational neuroanatomy

4. Morphology and intracortical projections of functionally characterised neurones in the cat visual cortex

5. A multimodal cell census and atlas of the mammalian primary motor cortex