Activity-dependent survival of odorant receptor neurons in ants

Abstract

ABSTRACTOlfaction is essential for complex social behavior in eusocial insects. To discriminate complex social cues, ants evolved an expanded number ofodorant receptor(Or) genes. Unlike most insect species, mutations in the obligate odorant co-receptor geneorcolead to loss of ∼80% antennal lobe glomeruli in ants. However, its cellular mechanism remains unclear. Here we demonstrate that this surprising neuronal phenotype results from massive apoptosis of odorant receptor neurons (ORNs) in the mid- to late-stages of pupal development. Further bulk and single-nucleus transcriptome analysis show that, although the majority oforco-expressing ORNs die inorcomutants, a small proportion of them survive: they expressionotropic receptor(Ir) genes that form IR complexes. In addition, we found that someOrgenes are expressed in mechanosensory neurons as well as non-neuronal cells, possibly due to the leaky regulation from nearby non-Orgenes. Our findings suggest that chemosensory receptors are required for activity-dependent survival of developing ORNs in ants.